ATP-Mg+2 Mediated Assembly of Rubisco Activase Investigated using Fluorescence Correlation Spectroscopy
نویسندگان
چکیده
منابع مشابه
Protein oligomerization monitored by fluorescence fluctuation spectroscopy: self-assembly of rubisco activase.
A methodology is presented to characterize complex protein assembly pathways by fluorescence correlation spectroscopy. We have derived the total autocorrelation function describing the behavior of mixtures of labeled and unlabeled protein under equilibrium conditions. Our modeling approach allows us to quantitatively consider the relevance of any proposed intermediate form, and K(d) values can ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2014
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2013.11.298